Imane Boudlal, 26, asked her employers at Disneyland's Grand Californian Hotel several months ago whether they would permit her to wear a head covering while working as a hostess, a spokeswoman for a workers' union said.
But when no reply was forthcoming, she decided to don the headscarf anyway, timing her decision with the beginning of the Muslim holy month of Ramadan, Leigh Shelton, a spokesman for the Unite Here Local 11 union said.
"Disney told Boudlal that if she wanted to work as a hostess she had to remove her hijab because it did not comply with the `Disney Look'," Shelton said.
"Disney further advised Boudlal that if she refused to remove her hijab, she could either work a back-of-the-house position where any customers would not see her, or else go home."
Boudlal refused the compromise and is now bringing Disney before the US Equal Opportunity Commission, a federal agency that handles claims of workplace discrimination.
"Their offer to put me in the back is humiliating," she said. "They're saying because I'm Arab, because I'm Moroccan, because I'm Muslim, they don't want to see me in the front."
The local branch of the Council on American-Islamic Relations, an advocacy group, said it had sent a letter to Disney demanding that the firm accommodate Boudlal.
"There is no justification for Disney's refusal to allow Ms Boudlal to wear her headscarf at work," said Ameena Mirza Qazi, the deputy executive director of the group and a staff lawyer.
"To say that her headscarf would somehow impact guests is not only insulting to her, but is deeply offensive to the thousands of Muslims who open up their pocket-books at Disney parks and resorts every year."